A gal4-driver line resource for drosophila neurobiology

 · The collection of GAL4 driver lines we describe here should be particularly useful for identifying and characterizing the morphology of neuronal populations in the adult fly for several reasons: (1) the lines express, on average, in fewer cells than the enhancer trap lines used in previous studies; (2) the number of lines we have imaged is larger than in any previous study; (3) we have imaged not Cited by: We established a collection of 7, transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord.  · To determine the expression patterns generated by each GAL4 driver line, we developed a high throughput pipeline for brain dissection, immunohistochemistry and imaging (see Experimental Procedures). Each GAL4 line was crossed to a UAS-GFP reporter line placing the expression of GFP under the positive control of GAL4 in the progeny, which were heterozygous for both the GAL4 driver Cited by:

The GAL4 lines have been deposited in the Bloomington Drosophila Stock Center for distribution and the expression patterns of the lines in several tissues are documented on a web-accessible database. We also developed a number of software tools to support our production pipeline and to analyze the large image dataset we generated; we expect these tools and methods to be useful for other large-scale imaging projects. We established a collection of 7, transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. A GAL4-driver line resource for Drosophila neurobiology. We established a collection of 7, transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer.

25 มี.ค. Drosophila brain has emerged as a powerful model system for the investigation A GAL4-driver line resource for Drosophila neurobiology. 19 ธ.ค. In Drosophila, the clock is controlled by only clock neurons that () A GAL4-driver line resource for Drosophila neurobiology. 30 พ.ค. A collection of split-GAL4 lines is generated that provides precise genetic targeting of different SEZ cell types in adult Drosophila.